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Part:BBa_K1859022:Experience

Designed by: Yusuke Banno   Group: iGEM15_Gifu   (2015-09-10)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1859022

[iGEM gifu 2015] .

We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane G shows this sample.




left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay

There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.


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